Coding

Part:BBa_K1129005

Designed by: UBC iGEM 2013   Group: iGEM13_British_Columbia   (2013-08-30)
Revision as of 02:13, 29 October 2013 by Fisal (Talk | contribs) (→‎Background)

Cinnamoyl-CoA reductase from Arabidopsis thaliana

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 201
    Illegal XhoI site found at 91
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 303
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

ATCCR1 is a Cinnamoyl CoA reductase found in Artabidopsis thaliana it functions to reduce the cinnamoyl coA molecule into cinnamaldehyde by utilizing the favourable kinetics offered by releasing the coA group. This enzyme belongs to the oxioreductase family and is involved in both the phenylpropanoid pathway and thus in the degradation of lignin.

===Experimental Data=== ----

https://static.igem.org/mediawiki/2013/2/2a/Const._EncP_%2B_AtCCR1.jpg

'''Figure 1'''. Compound generation identification by GC-MS. Chromatograms (left) and mass spectra (right) for select peaks are shown. Structures represent predictions based on library matching or comparison to standards. Controls represent plasmids missing the gene of interest. Top) GC-MS of a Cinnamic acid (10.580) control. Bottom) Conversion of Phenylalanine to Cinnamaldehyde (11.819) through the cinnamic acid intermediate(10.580) via the EncP, 4CL, and ATCCR1 gene construct under a constitutive promoter. The mass spec data shows a highly oxidized cinnamaldehyde that may have resulted from poor sample preparation ==Resources== http://www.uniprot.org/uniprot/Q1H8P3 http://www.arabidopsis.org/servlets/TairObject?id=137093&type=locus http://www.genome.jp/kegg-bin/show_pathway?map00940

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