Coding

Part:BBa_K1189034

Designed by: Taylor Remondini   Group: iGEM13_Calgary   (2013-10-24)
Revision as of 01:20, 29 October 2013 by TRemondini (Talk | contribs)

J04500_GFP_freiburg

This is a the standard GFP reporter (<a href="https://parts.igem.org/Part:BBa_E0040">E0040</a>) that contains the prefix/suffix required for RFC 25 standard assembly (<a href="https://parts.igem.org/Part:BBa_K648013">K648013</a>) placed behind an IPTG inducible promoter (<a href="https://parts.igem.org/Part:BBa_J04500">J04500</a>). This part allows for the easy construction and expression of fusion proteins containing GFP based on the Freiburg fusion standard (Muller et al,, 2007). Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


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