Generator
EHEC_TALE1

Part:BBa_K1189032:Design

Designed by: Ali Honarmand   Group: iGEM13_Calgary   (2013-09-27)
Revision as of 01:20, 29 October 2013 by Kate (Talk | contribs)

EHEC TALE1 (binds to 19bp of stx2 gene)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2404
    Illegal BamHI site found at 2539
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A KasI restriction cut site is incorporated at the end of the part so that we can swap the TALEs we currently have in our parts with this one.


Source

This part was synthesized IDT G-blocks and is in the process of being assembled together.

References

  • Meckler, J.F., Bhakta, M.S., Kim, M.S., Ovadia, R., Habrian, C.H., Zykovich, A., ... Baldwin, E.P. (2013). Quantitative analysis of TALE-DNA interactions suggests polarity effects. Nucleic Acids Research, 41(7), 4118–28. doi:10.1093/nar/gkt085
  • Heckman, K. L., & Pease, L. R. (2007). Gene splicing and mutagenesis by PCR-driven overlap extension. Nature protocols, 2(4), 924-932.
  • Perna, N. T., Plunkett, G., Burland, V., Mau, B., Glasner, J. D., Rose, D. J., ... Blattner, F. R. (2000) Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Letters to Nature. 409, 529-533. doi:10.1038/35054089