Reporter

Part:BBa_K1088007

Designed by: Andreas Kjær   Group: iGEM13_SDU-Denmark   (2013-08-20)
Revision as of 23:59, 3 October 2013 by P.R.A (Talk | contribs)

E. coli dxs-GFP fusion (lac promoter without lac inhibitor: IPTG uninducible)

This part consist of the dxs gene derived from E. coli fused to GFP at the translational level with a 10 AA linker between the proteins. The reporter fusion is under the control of the lac promoter and has a strong RBS.

The purpose of the part was to test the expression profile of dxs from the lac promter. BBa_K1088012 is a similar part that does not contain the linker-GFP part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2756
    Illegal SapI.rc site found at 946


[edit]
Categories
Parameters
None