Part:BBa_K1088000
1-deoxyxylulose-5-phosphate synthase (Dxs) enzyme from MEP pathway isolated from B. subtilis 168
The MEP pathway is a common bacterial isoprenoid pathway. To improve the yield of the end product, isopentenyl diphosphate (IPP) and dimethylallyl pyrophosphate(DMAPP), Zhao et al. expressed, in 2011, the 1-deoxyxylulose-5-phosphate synthase(Dxs) gene as well as the enzyme catalyzing the second step of the MEP pathway. They observed a 2.3 fold increase in isoprene output (isoprene is one of many products from the MEP pathway).
From Biocyc: "Dxs catalyzes the thiamin diphosphate-dependent reaction that condenses pyruvate and D-glyceraldehyde-3-phosphate to yield 1-deoxy-D-xylulose 5-phosphate [Julsing07]. This is the first, rate-limiting step in the methylerythritol phosphate pathway of isoprenoid biosynthesis, and is believed to feed into the pyridoxal 5-phosphate (vitamin B6) and thiamin (vitamin B1) biosynthesis pathways." -http://biocyc.org/BSUB/NEW-IMAGE?type=ENZYME&object=BSU24270-MONOMER
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1063
Illegal EcoRI site found at 1720
Illegal PstI site found at 1121
Illegal PstI site found at 1567 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1063
Illegal EcoRI site found at 1720
Illegal PstI site found at 1121
Illegal PstI site found at 1567 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1063
Illegal EcoRI site found at 1720 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1063
Illegal EcoRI site found at 1720
Illegal PstI site found at 1121
Illegal PstI site found at 1567 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1063
Illegal EcoRI site found at 1720
Illegal PstI site found at 1121
Illegal PstI site found at 1567
Illegal NgoMIV site found at 1020
Illegal AgeI site found at 913 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 862
Illegal SapI.rc site found at 1561
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