Part:BBa_K1129002

Enoyl-CoA hydratase/aldolase (ECH) from Pseudomonas putida

This is a enoyl-CoA hydratase/aldolase from Pseudomonas putida KT2440 which converts feruloyl-CoA to vanillin

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 755
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 184
  Illegal NgoMIV site found at 202
  Illegal NgoMIV site found at 241
  Illegal AgeI site found at 178
  
• 1000
  COMPATIBLE WITH RFC[1000]

DATA

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Figure 1 Compound generation identification by GC-MS. Chromatograms(left) and mass spectra(Right) for select peaks are shown. Structures represent predictions based on library matching or comparison to standards. Controls represent plasmids missing the gene of interest. A) Internal control using vanillin(10.486). B) Conversion of Ferulic acid to vanillin acid by constitutive expressed Fcs and Ech through the Feuroyl CoA intermediate, not shown. C) Conversion of Ferulic acid to vanillin acid by Arabinose inducible expressed Fcs and Ech through the Feuroyl CoA intermediate, not shown.