Coding

Part:BBa_K1031922

Designed by: He Shuaixin   Group: iGEM13_Peking   (2013-10-22)
Revision as of 16:19, 22 October 2013 by Psyche (Talk | contribs)

J23114-XylB-Terminator

For detailed information concerning XylB, please visit 2013 Peking iGEM Adaptors


Characterization

We achieved the concept of Adaptor by connecting XylC with XylS, and we want to further confirm whether other degradation enzymes could be added. So we construct XylB adaptor circuit adopting J23114 as constitutive promoter and B0015 as terminator (Fig 1)

Fig 1 Construction of adaptor circuit Pc/J23109-XylB. The orange arrowhead refers to constitutive Pc promoter. RBS is shown in green oval. The square in dark red stands for terminator B0015.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 364


Data shown

(Fig 2).

Fig 2



[edit]
Categories
//cds/enzyme
//chassis/prokaryote/pputida
//function/degradation
//terminator/double
Parameters
biology