Regulatory

Part:BBa_K1045005:Design

Designed by: iGEM Team Göttingen 2013   Group: iGEM13_Goettingen   (2013-09-20)
Revision as of 18:17, 17 October 2013 by Kati (Talk | contribs) (→‎Source)

YdaO Riboswitch with native RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into EcoRI and PstI digested pSB1C3 backbone.

Source

This part was generated by PCR amplification of wild type B. subtilis chromosomal DNA (strain B. subtilis 168; Laboratory collection AG StĂŒlke, Department for General Microbiology, University Göttingen).

References

Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965