Composite

Part:BBa_K1119011

Designed by: Wai In Hui   Group: iGEM13_Hong_Kong_HKUST   (2013-10-24)
Revision as of 11:59, 26 October 2013 by Hjleeab (Talk | contribs)

Human Elongation Factor-1alpha Promoter - GFP - hGH polyA tail

This is a mammalian GFP generator used for characterization of our EF-1alpha promoter (BBa_K1119010). This GFP generator contains the EF-1alpha promoter sequence with GFP reporter in RFC25 format(BBa_K648013) and hGH polyA terminator (BBa_K404108).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
    Illegal AgeI site found at 83
    Illegal AgeI site found at 1067
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1470
    Illegal BsaI.rc site found at 972


[edit]
Categories
Parameters
None