Coding
tetA(C)f

Part:BBa_J31007:Design

Designed by: Sabriya Rosemond, Erin Zwack   Group: iGEM06_Davidson   (2006-07-12)
Revision as of 18:07, 11 August 2006 by Macampbell (Talk | contribs) (→‎Design Notes)


tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 290
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 316
    Illegal NgoMIV site found at 684
    Illegal NgoMIV site found at 844
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was cloned into pSB1A2 plamsid. This sequence was amplified from pSB1A3 and is identical to the TetR gene found in pBR322. It turns out, this tetR gene actually has two open reading frames encoding two different proteins.

Source

PsB1AT3

References