Part:BBa_M36661

E. coli fur protein

The fur protein is found in E. coli and is involved in iron regulation. The protein dimerizes in the presence of iron, thus inhibiting transcription. At the end of the fur protein, we included a his-tag to be used as the primary antibody binding site in a Western blot assay. The his-tag is also followed by a stop codon.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 298
  Illegal PstI site found at 73
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 298
  Illegal PstI site found at 73
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 298
  Illegal BglII site found at 108
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 298
  Illegal PstI site found at 73
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 298
  Illegal PstI site found at 73
  
• 1000
  COMPATIBLE WITH RFC[1000]

Fully annotated primary DNA/RNA sequence: (note mutations and changes made to original sequence) (start codon included in bicistronic design RBS:)ACTGACAACAACACTGCACTGAAAAAAGCTGGCCTGAAAGTAACCCTTCCTCGCCTGAAAATCCTGGAAGTGCTGCAGGAACCGGACAACCACCACGTCAGCGCAGAAGATCTGTACAAACGCCTGATCGACATGGGGGAGGAGATCGGTCTGGCAACCGTCTATCGTGTTTTGAATCAGTTTGACGACGCAGGTATCGTGACCCGCCACAACTTCGAAGGCGGTAAAAGCGTCTTTGAACTGACCCAACAGCACCACCACGATCACCTGATCTGCCTGGACTGCGGCAAAGTGATCGAATTCAGCGACGATAGTATTGAAGCACGTCAGCGTGAAATCGCTGCAAAGCACGGTATCCGTTTGACGAACCACAGCCTGTATCTTTACGGCCATTGTGCCGAAGGTGATTGCCGTGAAGATGAGC