Protein_Domain

Part:BBa_M36661

Designed by: Frankie Willcox, Claudia Dennler, Meelim Lee   Group: Stanford BIOE44 - S11   (2013-10-25)
Revision as of 08:06, 25 October 2013 by Registry (Talk | contribs)

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E. coli fur protein

The fur protein is found in E. coli and is involved in iron regulation. The protein dimerizes in the presence of iron, thus inhibiting transcription. At the end of the fur protein, we included a his-tag to be used as the primary antibody binding site in a Western blot assay. The his-tag is also followed by a stop codon.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 73
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 73
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 298
    Illegal BglII site found at 108
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 73
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 298
    Illegal PstI site found at 73
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None