Part:BBa_K1129050

Cinnamoyl-CoA reductase from Arabidopsis thaliana under arabinose promoter

ATCCR1 is a Cinnamoyl CoA reductase found in Artabidopsis thaliana it functions to reduce the cinnamoyl coA molecule into cinnamaldehyde by utilizing the favourable kinetics offered by releasing the coA group. This enzyme belongs to the oxioreductase family and is involved in both the phenylpropanoid pathway and thus in the degradation of lignin.

Applications of BBa_K1129050

Experience

Figure 1. Compound generation identification by GC-MS. Chromatograms (left) and mass spectra (right) for select peaks are shown. Structures represent predictions based on library matching or comparison to standards. Controls represent plasmids missing the gene of interest. Top) GC-MS of a Cinnamic acid (10.580) control. Bottom) Conversion of Phenylalanine to Cinnamaldehyde (11.819) through the cinnamic acid intermediate(10.580) via the EncP, 4CL, and ATCCR1 gene construct under a constitutive promoter. The mass spec data shows a highly oxidized cinnamaldehyde that may have resulted from poor sample preparation

User Reviews

UNIQedaac8e2c4792e69-partinfo-00000001-QINU UNIQedaac8e2c4792e69-partinfo-00000002-QINU Resources

http://www.uniprot.org/uniprot/Q1H8P3

http://www.arabidopsis.org/servlets/TairObject?id=137093&type=locus

http://www.genome.jp/kegg-bin/show_pathway?map00940

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 357
  Illegal BamHI site found at 65
  Illegal XhoI site found at 247
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 459
  
• 1000
  COMPATIBLE WITH RFC[1000]