Part:BBa_K1045000

DarR operator

This part is the binding sequence of the c-di-AMP-dependent regulatory protein DarR. When bound by DarR, it is supposed to prevent the downstream information to be transcribed.
The important information: The operator is not in the pSB1C3 Vector but instead in the pBluescript-KS plasmid. This plasmid provides an ampicillin resistance. Furthermore, it has got a pUC origin.

The DarR operator is a 14 bp palindromic DNA sequence discovered in Mycobacterium smegmatis. In M. smegmatis, the DarR operator is found upstream of darR (auto-regulation) and other genes. The sequence motif of the DarR operator varies slightly dependent on the downstream gene (Zhang et al., 2013). To create this biobrick, the operator sequence found upstream of darR was used.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]