Coding
sfGFPN

Part:BBa_K1093005

Designed by: Anna Miścicka   Group: iGEM13_Warsaw   (2013-09-20)
Revision as of 18:23, 12 October 2013 by Annamiscicka (Talk | contribs)

sfGFP N-terminal

Superfolder Green Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eGFP it displays improved stability and strong blue fluorescence. Excitation peak: 380 nm Emission peak: 440 nm. This is N-terminal of sfBFP, that can be used in Bimolecular Fluorescence Complementation system (BiFC). It can be used in conjunction with two versions of superfolderFP C-terminus (BBa_K1093014 and BBa_K1093015) of different specificity to sensitivity ratio (BBa_K1093007 being the more sensitive and BBa_K1093008 the more specific). The 'wild type' C-terminus is not applicable, as it spontaneously recreates the native fluorophore with the N-terminal part. Part contains Freiburg prefix (suitable for fusion at the N-terminus).

References

1: Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006 Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006 Sep;24(9):1170. PubMed PMID: 16369541.

2: Zhou J, Lin J, Zhou C, Deng X, Xia B. An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein. Acta Biochim Biophys Sin (Shanghai). 2011 Mar;43(3):239-44. doi: 10.1093/abbs/gmq128. Epub 2011 Jan 27. PubMed PMID: 21273204.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


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Categories
Parameters
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