Composite

Part:BBa_K1055007

Designed by: Sven Jager, Sebastian Wagner and Anne Schieferdecker   Group: iGEM13_TU_Darmstadt   (2013-09-18)
Revision as of 13:32, 18 September 2013 by Registry (Talk | contribs)

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FsC: functional cutinase

The part enables the user to produce functional cutinase from fungus Fusarium solani pisi which is a cuticula degrading hydrolase. The enzyme is fused to mRFP to allow tracking of expression and also provides a His-Tag for subsequent purification. Both, mRFP and His-Tag, can be cleaved using TEV-Protease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NotI site found at 587
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 733
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 190
    Illegal AgeI site found at 1420
    Illegal AgeI site found at 1532
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 509


[edit]
Categories
Parameters
None