![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K1055007
Designed by: Sven Jager, Sebastian Wagner and Anne Schieferdecker Group: iGEM13_TU_Darmstadt (2013-09-18)
FsC: functional cutinase
The part enables the user to produce functional cutinase from fungus Fusarium solani pisi which is a cuticula degrading hydrolase. The enzyme is fused to mRFP to allow tracking of expression and also provides a His-Tag for subsequent purification. Both, mRFP and His-Tag, can be cleaved using TEV-Protease.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NotI site found at 587 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 733
Illegal BamHI site found at 65 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 190
Illegal AgeI site found at 1420
Illegal AgeI site found at 1532 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 509
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Categories
Parameters
None |