Temporary

Part:BBa_J25500:Design

Designed by: Mackenize Cowell   Group: iGEM06_BU   (2006-07-12)
Revision as of 00:06, 13 July 2006 by Registry (Talk | contribs)

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Light-repressed mCherry (uses photosensor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1702
    Illegal BglII site found at 4190
    Illegal BamHI site found at 65
    Illegal XhoI site found at 2297
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1255
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Question: in M30109, why is i15010 controlled by a separate promoter from i15008, i15009? Could they all be contiguous and controlled by a single promoter?


Source

The photosensor (M30109) Natalie Kuldell used in her BE.109 course & mCherry+lva (J06505).

References