Coding

Part:BBa_K1223013:Design

Designed by: Orr Schlesinger   Group: iGEM13_BGU_Israel   (2013-10-03)
Revision as of 16:10, 3 October 2013 by Schlez (Talk | contribs) (→‎Source)


Pyrolysyl-tRNA synthetase CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 394
    Illegal BglII site found at 755
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 90
    Illegal BsaI.rc site found at 128


Design Notes

CDS of the pyrolysyl-tRNA synthetase (pylS) that charges the pyrolysine tRNA (pylT) with pyrrolysine.

Source

derived from the genomic sequence of Methanosarcina barkeri. gene name - pylS

clasification: Archaea : Euryarchaeota : Methanomicrobia : Methanosarcinales : Methanosarcinaceae : Methanosarcina

References

Srinivasan,G., James,C.M. and Krzycki,J.A., Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA, Science 296 (5572), 1459-1462 (2002)

Milles, S.; Tyagi, S.; Banterle, N.; Koehler, C.; VanDelinder, V.; Plass, T.; Neal, A. P.; Lemke, E. A., Journal of the American Chemical Society 2012, 134 (11), 5187-95.