![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1159316
Designed by: TU Munich 2013 Group: iGEM13_TU-Munich (2013-09-16)
SERK-Receptor TMD with C-terminal GFP and N-terminal Strep-tag II TEV-site linker in RFC[25]
This part codes for the Transmembrane Domain of SERK Receptor from Physcomitrella patens with a C-terminal (intracellular) GFPmut1 fusion and a N-terminal fusion to a linker containing Strep-tag II and a TEV cleavage site; SERK TMD and GFPmut1 are seperated by a middle long linker. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
Note: A signal peptide is needed for membrane integration.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 8
Illegal BsaI.rc site found at 926
[edit]
Categories
Parameters
//chassis/eukaryote/ppatens
None |