![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1159112
Designed by: Dong-Jiunn Jeffery TRUONG Group: iGEM13_TU-Munich (2013-09-16)
FRET Reporter for TEV Protease activity (eCFP_TEV-site-linker_SYFP2) in RFC[25]
This part encodes a FRET reporter with the chromophores eCFP and SYFP2 that is seperated by a TEV cleavage site. As a FRET pair, excitation of eCFP leads to a energy transfer from eCFP to SYFP2 (FRET) resulting in a fluorescence of SYFP2. In the presence of TEV Protease cleavage actitivy the FRET pair SYFP2 is cleaved off from eCFP and eCFP is not anymore quenched by SYFP2 resulting in the fluorescence of eCFP. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |