Part:BBa_K1159311

GFPmut1

This part codes for GFPmut1 which is flanked by RFC[25] pre- and suffix.

Protein data table for BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_">BBa_</a> automatically created by the <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">BioBrick-AutoAnnotator</a> version 1.0

Nucleotide sequence in RFC 25 N-Part using the stop codon in the suffix, so ACCGGT was added (in italics) to the 3' end: (underlined part encodes the protein)
AGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGACG ... AGGGTGAAGACCGGT
ORF from nucleotide position 53 to 106 (excluding stop-codon)

Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)

1	MVTLMGTNFLSVERVKTG*

Sequence features: (with their position in the amino acid sequence, see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software/FeatureList">list of supported features</a>)

None of the supported features appeared in the sequence

Amino acid composition:

Ala (A)	0 (0.0%)
Arg (R)	1 (5.6%)
Asn (N)	1 (5.6%)
Asp (D)	0 (0.0%)

Cys (C)	0 (0.0%)
Gln (Q)	0 (0.0%)
Glu (E)	1 (5.6%)
Gly (G)	2 (11.1%)

His (H)	0 (0.0%)
Ile (I)	0 (0.0%)
Leu (L)	2 (11.1%)
Lys (K)	1 (5.6%)

Met (M)	2 (11.1%)
Phe (F)	1 (5.6%)
Pro (P)	0 (0.0%)
Ser (S)	1 (5.6%)

Thr (T)	3 (16.7%)
Trp (W)	0 (0.0%)
Tyr (Y)	0 (0.0%)
Val (V)	3 (16.7%)

Amino acid counting

	Total number:	18
	Positively charged (Arg+Lys):	2 (11.1%)
	Negatively charged (Asp+Glu):	1 (5.6%)
	Aromatic (Phe+His+Try+Tyr):	1 (5.6%)

Biochemical parameters

	Atomic composition:	C₈₆H₁₄₇N₂₃O₂₆S₂
	Molecular mass [Da]:	1983.4
	Theoretical pI:	8.50
	Extinction coefficient at 280 nm [M^-1 cm^-1]:	0 / 0 (all Cys red/ox)

Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges <input type='button' id='hydrophobicity_charge_button' onclick='show_or_hide_plot_1381237866743()' value='Show'>

Codon usage

	Organism:	E. coli	B. subtilis	S. cerevisiae	A. thaliana	P. patens	Mammals
	Codon quality (<a href="http://en.wikipedia.org/wiki/Codon_Adaptation_Index">CAI</a>):	good (0.74)	good (0.75)	good (0.61)	good (0.68)	excellent (0.83)	good (0.74)

Alignments (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)
There were no alignments for this protein in the data base. The BLAST search was initialized and should be ready in a few hours.

Predictions (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)

There were no predictions for this protein in the data base. The prediction was initialized and should be ready in a few hours.

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If you have any questions, comments or suggestions, please leave us a <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">comment</a>.

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Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 641