Coding

Part:BBa_K302010

Designed by: Harsh Sheth, Alan Koh, Steven Woodhouse   Group: iGEM10_Newcastle   (2010-08-12)
Revision as of 15:37, 28 September 2010 by Swoodhouse (Talk | contribs)

sfp

Bacillus subtilis 168 is unable to produce surfactin, a natural surfactant which helps in reducing surface tension and aids bacteria to swarm on a solid or semi-solid medium. This is because the 168 chromosome contains a frameshift mutation in the gene sfp.

This part is an intact sfp which restores the capability to produce surfactin.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 651
    Illegal SapI.rc site found at 661


[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
None