Coding
Part:BBa_K1113701:Experience
Designed by: Belen Cespedes Group: iGEM13_UC_Chile (2013-09-18)
Applications of BBa_K1113701
We test the targeting sequence of the RuBisCO-GFP (the large and short subunit) in a co-transformation with the Carboxysome biobick (Bba_K1113100)+ RFP, all this in the TOP10 E. coli strain, then we observe this bacterias with the confocal microscope and look for colocalization of both GFP (targeting sequence) and RFP (carboxysomes).
Figure 1. Confocal Microscopy. Brightfield, GFP filter, RFP filter and merge are shown
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