Coding

Part:BBa_K1104301

Designed by: Jou-Chien Liao, Rohan Shina, Yucheng Huang   Group: iGEM13_NYMU-Taipei   (2013-09-11)
Revision as of 05:55, 5 October 2013 by JRDeath (Talk | contribs)

Defensin1

　　Defensin is a grand group of antimicrobial peptides, having numerous kinds of similar AMPs. They are labeled by numbers, from species to species, as their identification. Different from well-studied Defensins secreted from human, our Defensin1 from honey bee has many unclear properties. Even so, its effectiveness against microbes has been proved through some stable papers.

　　Importantly, the sequence here is the mature peptide sequence because there are some introns which are barriers for E. coli to transcript genes in the genome DNA. That is to say, it is better to synthesize the gene through designing primers(about two sets >> four primers) as modules for real PCR. Comparatively, purifying genes & proteins from bees' genome DNA extraction is a more complicated and time-consuming method.

Working Mechanism

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 135
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None