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Part:BBa_K1216002:Design

Designed by: Parvathi Chandran   Group: iGEM13_ETH_Zurich   (2013-08-29)
Revision as of 03:51, 5 October 2013 by Sabine.oesterle (Talk | contribs) (→‎Design Notes)

Acetyl esterase (aes) from Escherichia Coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 951


Design Notes

NO design considerations.



We obtain the plasmid with the hydrolase from Johannes Haerle of the Panke Group from D-BSSE in Basel and checked them for restriction sites and sequenced them to confirm the sequence. We did PCR with overlapping fragments to introduce the biobrick pre and suffix to clone them into biobrick vectors.

Source

Escherichia coli

References