Generator
Part:BBa_K1216002:Design
Designed by: Parvathi Chandran Group: iGEM13_ETH_Zurich (2013-08-29)
Acetyl esterase (aes) from Escherichia Coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 27
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 951
Design Notes
NO design considerations.
We obtain the plasmid from Johannes Haerle of the Panke Group from D-BSSE in Basel and sequence them for restriction sites and further cloning steps
Source
Escherichia coli