Generator

Part:BBa_K1216002:Design

Designed by: Parvathi Chandran   Group: iGEM13_ETH_Zurich   (2013-08-29)
Revision as of 03:44, 5 October 2013 by Blockmoka (Talk | contribs)

Acetyl esterase (aes) from Escherichia Coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 951


Design Notes

NO design considerations.



We obtain the plasmid from Johannes Haerle of the Panke Group from D-BSSE in Basel and sequence them for restriction sites and further cloning steps


Source

Escherichia coli

References