Composite

Part:BBa_K1197009

Designed by: Emre İlpars   Group: iGEM13_METU_Turkey   (2013-09-22)
Revision as of 03:42, 5 October 2013 by Alisankayabolen (Talk | contribs)

Phyp + RBS (SpoVG) + RFP + DT

This part is used together with K119008 and K1197010 to compare two Pveg promoter (K143012 and K823003) and one pHyperspank promoter (K606040) which used in B.subtilis and have different sequences. By RFP production and modelling data, strength of promoters were compared.

.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 673
    Illegal AgeI site found at 785
  • 1000
    COMPATIBLE WITH RFC[1000]

IPTG = 0

K606040_-_IPTG_%3D_0.jpg

IPTG = 5000

K606040_-_IPTG_%3D_5000.jpg


[edit]
Categories
//chassis/prokaryote/bsubtilis
//function/reporter/fluorescence
Parameters
None