Generator

Part:BBa_K1060003:Experience

Designed by: Ingmar Claes, Robbert Boudewijns, Laurens De Backer   Group: iGEM13_KU_Leuven   (2013-09-17)
Revision as of 22:57, 4 October 2013 by Dinox (Talk | contribs) (→‎Improvement of BBa_J45700)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Characterization of BBa_K1060003

BBa_K1060003.jpg

E. coli BL21(DE3) strain

IPTG: 0,2mM

salicylate: 1mM

chorismate: 1mM

control: BL21(DE3) without plasmid and antbiotics


System testing

Smell Test

BBa_K1060003_SmellTest_24h_37C.jpg


To test this device several setups for smell tests where made. In the graph shown here the samples were incubated for 24h at 37°C. Methyl salicylate (MS) has a wintergreen odor and can be detected by scent. 11 people smelled each sample independently of one another and answered if they could smell MS or not. For more details on the experimental setup and a discussion of the results go [http://2013.igem.org/Team:KU_Leuven/Project/Glucosemodel/MeS here] under 'smell test'. Chorismate was added to test the salicylate production (BBa_J45320) and salicylate to test the wintergreen odor generator (BBa_J45120).


Improvement of BBa_J45700

As we recloned this part into standard pSB1C3 backbone, in the sense of ease of use, we improved the brick BBa_J45700.

User Reviews

UNIQ2d75f7859d42c2e0-partinfo-00000002-QINU UNIQ2d75f7859d42c2e0-partinfo-00000003-QINU

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Sander Wuyts

The KU Leuven iGEM 2013 team tried to perform a qPCR on this part. It was impossible to remove the original plasmid DNA, after RNA isolation, even after several attempts with different DNase treatments. This problem is probably due to the fact that this part was provided in a high copy number backbone. If you want to perform a qPCR yourself, we recommend you to clone this part in another backbone or in the genome itself.

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