Regulatory

Part:BBa_K1163101

Designed by: Emiel van der Kouwe   Group: iGEM13_Evry   (2013-09-22)
Revision as of 19:43, 22 September 2013 by Eccho (Talk | contribs)

pAceB promoter region

Promoter from E. coli that controls the AceB gene, involved in iron uptake and iron sensitive.

FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription.

Why would you use this part?

This promoter allows you to have an iron-sensitive behaviour. In fact, in the presence of iron, the AceB promoter region decreases the expression of the downstream gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 277
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/sensor/metal
Parameters
None