Device

Part:BBa_K1150041

Designed by: Ilona Blank   Group: iGEM13_Freiburg   (2013-09-17)
Revision as of 23:19, 4 October 2013 by AnneM (Talk | contribs) (→‎Usage and Biology)

uniCAS RNaimer to Bla Target 3


RNaimer (Bla target 3)
Function Targeting VEGF3 with Cas9
Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]


This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered dCas9 protein to the Bla locus target 3 . This RNaimer is bulid of the uniCAS RNAimer and contains the DNA sequence coding for a crRNA that binds complementary to Bla 3 target.

Usage and Biology

Freiburg 2013 used this plasmid to test the efficiency of target Bla3 by activating or repressing a SEAP reporter gene.



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