Generator

Part:BBa_K1060009:Experience

Designed by: Ingmar Claes, Su Wang, Sylvie Weckx   Group: iGEM13_KU_Leuven   (2013-09-19)
Revision as of 22:10, 4 October 2013 by LMarcelis (Talk | contribs) (→‎System testing)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Characterization of BBa_K1060009

BBa_K1060009.jpg

E. coli BL21(DE3) strain

Mg2+: 5mM

control: BL21(DE3) without plasmid and antibiotics

System testing

[http://www.youtube.com/watch?v=n-pMp8-XdUo Aphid Repellence video]

In this video you will find our first behavioural experiment with aphids. An EBF-producing bacterium plate (E. coli BL21(DE3) on LB with 5mM Mg2+ and Chloramphenicol is placed on the left, a control plate on the right, aphids are placed on the leaf in the middle. The first results indicate a positive trend, as we can see them moving from left to right. (You may have to watch the video in full screen to clearly see the aphids.) For more information on the experimental setup and a discussion of the results go to [http://2013.igem.org/Team:KU_Leuven/Project/Glucosemodel/EBF#aphidexperiments here] and scroll down to Aphid experiments.

User Reviews

UNIQe722f7ef2a765e6b-partinfo-00000000-QINU UNIQe722f7ef2a765e6b-partinfo-00000001-QINU