Deleted
Sample Discontinued
Experience: None
Not Used
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Tag
ccdB_Ind

Part:BBa_K1152007

Designed by: Joshua Sachs   Group: iGEM13_Heidelberg   (2013-09-20)
Revision as of 21:38, 4 October 2013 by Joshua (Talk | contribs)

Helper construct for NRP-Indigoidine-tagging

This part serves as a template for the labeling of non-ribosomal peptides with indigoidine. The desired order of modules can be used to replace the suicide gen ccdB. Therefore, selection of positive transformed clones gets a lot easier and nascent non-ribosomal peptides are tagged by the Indigoidine residue attached during synthesis. Since the blue pigment Indigoidine can be seen without auxillary means, analytical procedures can be faciliated a lot. Instead of complex labeling, tagging or work up procedures, simple devices, like a TLC can be used to asses qualitativ differences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3061
    Illegal BamHI site found at 891
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 341
    Illegal SapI.rc site found at 4324


[edit]
Categories
//proteindomain
Parameters
n/aHelper construct for NRP-Indigoidine-tagging