Coding

Part:BBa_K1152000

Designed by: Tania Christiansen   Group: iGEM13_Heidelberg   (2013-09-16)
Revision as of 14:33, 16 September 2013 by Registry (Talk | contribs)

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TycA_deltaCom Module from Tyrocidine

This is the CDS for TycA_deltaCom module from the Tyrocidine gene cluster of Brevibacillus Parabrevis. It is the first module in this cluster for the NonRibosomalPeptide-Synthetase of the cyclic decamer Tyrocidine. This specific module Tyca_deltaCom is responsiple for the binding of the first amino acid D-Phenyl of the NRP. It consists of the A- (adenylation), PCP- (peptide carrier protein) and the E- (epimerization) domain. After Tyc A follows the TycB gene with three modules.

This bio brick can be used for the design of different NRPS. It was used in the synthetic Tripeptide I of our project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2518


[edit]
Categories
//cds/biosynthesis
Parameters
biology