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Translational_Unit

Part:BBa_K1122676

Designed by: Aleksandra Lewicka, Jan Lyczakowski   Group: iGEM13_Edinburgh   (2013-08-30)
Revision as of 19:09, 4 October 2013 by JanL (Talk | contribs)

IPTG inducible pdc and adhB

This parts enables expression of pyruvate decarboxylase (pdc) and alcohol dehydrogenase B (adhB) using IPTG induction. Those enzymes are involved in conversion of pyruvate to ethanol via an acetaldehyde intermediate. The pdc and adhB sequences come from Zymomonas mobilis however, they are codon optimised for E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1109
    Illegal AgeI site found at 2315
  • 1000
    COMPATIBLE WITH RFC[1000]


References

INGRAM, L. O., CONWAY, T., CLARK, D. P., SEWELL, G. W. & PRESTON, J. F. 1987. GENETIC-ENGINEERING OF ETHANOL-PRODUCTION IN ESCHERICHIA-COLI. Applied and Environmental Microbiology, 53, 2420-2425.

[edit]
Categories
//cds/biosynthesis
//cds/enzyme
Parameters
None