Regulatory

Part:BBa_K1123000

Designed by: Ardjan van der Linden   Group: iGEM13_TU-Eindhoven   (2013-08-31)
Revision as of 18:39, 4 October 2013 by ArdjanvanderLinden (Talk | contribs)

FNR Promoter

This part is a standard FNR promoter. It has two FNR binding sites at -41.5 and at -91.5 with 0 being the transcriptional starting point of this promoter. The promoter can be used in E.coli strains to induce protein expression in anaerobic environments. It is natively used to induce metabolic processes when the E.coli bacteria enter anaerobic environments. The part can be used to express any protein sequence placed behind it.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 39
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

This part is one of the most essential parts used within the 2013 Tu-Eindhoven iGEM project.


[edit]
Categories
Parameters
None