Composite

Part:BBa_K1123001

Designed by: Ardjan van der Linden   Group: iGEM13_TU-Eindhoven   (2013-09-08)
Revision as of 11:53, 8 September 2013 by Registry (Talk | contribs)

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Protamine-1-Optimized Protein Production Construct

This composite part has been built up from a number of separate BioBricks, the most prominent of which the FNR promotor and the Protamine-1-Optimized Protein sequence. The FNR promotor sequence enables us to bring the Protamine-1-Optimized protein to expression anaerobically. Almost all E.coli strains posses a form of this promotor to induce metabolic processes once the bacteria enters anaerobic regions. The Protamine-1-Optimized protein is a human protein which we are expressing with the sole purpose of using it as a CEST contrast agent.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 39
    Illegal XhoI site found at 359
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None