Coding
Part:BBa_K1152001:Design
Designed by: Tania Christiansen Group: iGEM13_Heidelberg (2013-09-16)
TycB1 Module from Tyrocidine
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1195
Design Notes
Silent mutation was found after sequencing with VR and VF2 at positions 4595 (GCC to GCG; Alanin to Alanin) and at position 4598 (CTT to CTG; Leucine to Leucine).
Source
Tyrocidine Gene Cluster of Brevibacillus Parabrevis. Genebank File taken from http://www.ncbi.nlm.nih.gov/nuccore/af004835