Device

Part:BBa_K1150032

Designed by: Freiburg 2013   Group: iGEM13_Freiburg   (2013-09-17)
Revision as of 12:28, 4 October 2013 by Ilona (Talk | contribs)

uniCAS UV Light Switch Part II - Histone Modifier

CMV:COP1-G9a:BGH
Function Histone modification domain

of UVB light induced gene

expression control

Use in Mammalians
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]


Introduction

This part contains the protein COP1 fused to the Methyl-Tranferase G9a. It's an interaction partner of dCas9-UVR8 and part of the UVB light controlled mechanism to repress gene expression. You will be able to repress any desired gene by a short UV-light pulse.

Biology & Usage

COP1 is a protein that was first described in the model organism Arabidopsis thaliana. It will bind to the UV-light receptor UVR8 after illumination [1]. Thereby recruiting G9a to its target locus. Subsequently the local DNA-expression will be repressed by DNA-methylation. For this UVB light controled gene repression system you need a combination of these BioBricks: Cas9-UVR8, COP1-G9a and RNaimer.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BglII site found at 2231
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1373
    Illegal BsaI.rc site found at 1450
    Illegal SapI.rc site found at 962

References

(1) Muller, K.; Engesser, R.; Schulz, S.; Steinberg, T.; Tomakidi, P.; Weber, C. C. et al. (Nucleid Acids Research 2013): Multi-chromatic control of mammalian gene expression and signaling


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