Part:BBa_K1150032
uniCAS UV Light Switch Part II - Histone Modifier
uniCAS UV Light Switch
Part II - Histone Modificator | |
---|---|
Function | Histone modification domain
of UVB light induced gene expression control |
Use in | Mammalians |
RFC standard | RFC 25 |
Backbone | pSB1C3 |
Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
Introduction
This part contains the protein COP1 fused to the Methyl-Tranferase G9a. Together with the UV-light switch Part I, you will be able to repress virtually any desired gene indicated by a short UV-light pulse. COP1 is a protein that was first described in the model organism Arabidopsis thaliana. It will bind to the UV-light receptor UVR8 after illumination. Thereby recruiting G9a to its target locus. Subsequently the local DNA-expression will be repressed by DNA-methylation
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
Illegal BglII site found at 2231 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1373
Illegal BsaI.rc site found at 1450
Illegal SapI.rc site found at 962
References
Muller, K.; Engesser, R.; Schulz, S.; Steinberg, T.; Tomakidi, P.; Weber, C. C. et al. (Nucleid Acids Research 2013): Multi-chromatic control of mammalian gene expression and signaling
None |