Tag
Si4

Part:BBa_K1028000

Designed by: Oleg Karpov, Joseph Beton   Group: iGEM13_Leeds   (2013-08-30)
Revision as of 12:07, 4 October 2013 by Joebeton (Talk | contribs)

Silica binding peptide

Introduction

This BioBrick is a short peptide of 15 amino acids designed to act as a tag binding with high affinity to silica and silica-like particles. As a tag it can easily be added to the C-terminus of proteins using molecular biology techniques allowing the peptide to assist with purification or to serve other experimental processes related to silica binding.
http://i276.photobucket.com/albums/kk29/JosephBeetBeet/d9a54a14-fa9a-4957-b11b-95d7323de4bf.jpg
This part was originally designed by the Leeds 2013 iGEM team to bind with silica microspheres, as shown schematically above, when presented on the exterior of the plasma membrane via fusion with Ice Nucleation Protein.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None