Part:BBa_K1150030

uniCAS UV Light Switch Part II - Activator

CMV:COP1-VP16:BGH
Function	Activation domain of UVB light induced gene expression control
Use in	Mammalians
RFC standard	RFC 25
Backbone	pSB1C3
Submitted by	[http://2013.igem.org/Team:Freiburg Freiburg 2013]

The fusion protein COP1-VP16 is an interaction partner of dCas9-UVR8. It's a part of the UVB light controlled mechanism to activate or repress gene expression. First the dCas9-UVR8 fusion protein binds specifically to a DNA target sequence. If the system is exposed to UVB light (311 nm), the UVR8 receptor of UV changes its configuration and recruits this COP1-VP16 protein. This activates gene expression induced by UVB light. For this UVB light controled gene activation system you need a combination of these BioBricks: Cas9-UVR8, COP1-VP16 and RNaimer.

Biology and Usage

E3-ubiquitin ligase Constitutively Photomorphogenic 1 (COP1) is the interaction partner of ultraviolet B (UVB) light receptor UVR8 [1]. When UVB light is perceived by the UVR8 photoreceptor it monomerizes and therefore changes in its open confuguration. Not till then it recruits COP1.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 576
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1373
  Illegal BsaI.rc site found at 1450
  Illegal SapI.rc site found at 962