Reporter

Part:BBa_K1073022:Experience

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)
Revision as of 12:35, 28 September 2013 by TUNKI (Talk | contribs) (Applications of BBa_K1073022)


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Applications of BBa_K1073022

The iGEM Team Braunschweig 2013 used this device as a reporter to distinguish two different strains when cultivation in a mixed culture.
When eforRed is expressed, the colonies on agar plates as well as the liquid culture exhibit a bright pink color.

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iGEM Team Braunschweig 2013

When expressed, the chromoprotein eforRed exhibits a distinct pink color. In order to avoid absorption by the chomoprotein during OD measurement of a bacterial culture, the absorption and emission spectra were measured. An optimal wavelength for spectral optical density measurement was determined to be at 520nm.

BSAbsorption-eforRed.jpg

iGEM Team Braunschweig 2013: Absorptionspectrum of chromoprotein eforRed in the wavelength range 400-800 nm.

Absorption maximum of eforRed: 534 nm

BS2013Emission eforRed.jpg

iGEM Team Braunschweig 2013: Emission spectrum of chromoprotein eforRed. The chromoprotein does show significant emission and can be detected by its fluorescence.

Emission maximum of eforRed: 605 nm


Fluorescence.JPG

iGEM Team Braunschweig 2013: Supernatant after cell disruption containing chromoproteins of eforRed and amilGFP (BBa_K1073024). Both chromoproteins fluoresce when excited by UV-light.

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