Translational_Unit

Part:BBa_K1065103

Designed by: Caterina Marchioretti Michele Pedrotti   Group: iGEM13_UNITN-Trento   (2013-08-28)
Revision as of 18:24, 3 October 2013 by Cridelbianco (Talk | contribs)

PchBA

This BioBrick contains the coding sequence for the two enzymes PchA and PchB. This part was submitted in pSB1C3 to the registry by the UniTN-Trento 2013, using an original part of iGEM MIT 2006 BBa_J45319 in pSB1AT3.

This part was cloned by the iGEM UniTN Trento 2013 team for the creation of Wintergreen Devices BBa_K1065102 and BBa_K1065106.

Usage and Biology

PchB is a isochorismate-pyruvate lyase while PchA is a isochorismate synthase, both from the microorganism Pseudomonas aeruginosa. These enzymes are responsible for the production of salicylate acid.

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
    Illegal NgoMIV site found at 730
    Illegal NgoMIV site found at 855
    Illegal NgoMIV site found at 866
    Illegal NgoMIV site found at 1147
    Illegal NgoMIV site found at 1615
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None