Coding
Part:BBa_K1033260
Designed by: Anders Edlund Group: iGEM13_Uppsala (2013-09-13)
Toxin-antitoxin system from Lactobacillus plantarum
This is a toxin-antitoxin system (pem) together with a natural putative promotor from the Lactobacillus plantarum NC7 plasmid p256. It has been shown to increase segregational stability under non-selective pressure. The system has experimentally been shown to allow 88-100% retention of a plasmid after 80 generations. See http://mic.sgmjournals.org/content/151/2/421.long.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 158
Illegal SpeI site found at 534 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 158
Illegal SpeI site found at 534 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 158
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 158
Illegal SpeI site found at 534 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 158
Illegal SpeI site found at 534 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |