Coding

Part:BBa_K1033260

Designed by: Anders Edlund   Group: iGEM13_Uppsala   (2013-09-13)
Revision as of 18:45, 13 September 2013 by Registry (Talk | contribs)

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Toxin-antitoxin system from Lactobacillus plantarum

This is a toxin-antitoxin system (pem) together with a natural putative promotor from the Lactobacillus plantarum NC7 plasmid p256. It has been shown to increase segregational stability under non-selective pressure. The system has experimentally been shown to allow 88-100% retention of a plasmid after 80 generations. See http://mic.sgmjournals.org/content/151/2/421.long.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 158
    Illegal SpeI site found at 534
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 158
    Illegal SpeI site found at 534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 158
    Illegal SpeI site found at 534
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 158
    Illegal SpeI site found at 534
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None