Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Translational_Unit

Part:BBa_K1122674

Designed by: Aleksandra Lewicka, Jan Lyczakowski   Group: iGEM13_Edinburgh   (2013-08-22)
Revision as of 13:47, 3 October 2013 by JanL (Talk | contribs) (→‎Functional Parameters)

Plac+fused PDC-ADH

lac promoter plus PDC-ADH fusion protein for ethanol production


Generation of protein fusion

Bioethanol1.jpg

Bioethanol2.jpg

Evidence for presence of protein fusion

DNA level evidence


Bioethanol3.jpg

File:Bioethanol sequencing.jpg


Protein level evidence


Bioethanol4.jpg

Bioethanol5.jpg

Bioethanol6.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1109
    Illegal AgeI site found at 2315
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

Bioethanol7.jpg

Activity of adhB as a fusion member


Bioethanol8.jpg

Microscopy of cells expressing fused protein


Bioethanol9.jpg

[edit]
Categories
Parameters
None