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Translational_Unit

Part:BBa_K1122674

Designed by: Aleksandra Lewicka, Jan Lyczakowski   Group: iGEM13_Edinburgh   (2013-08-22)
Revision as of 13:38, 3 October 2013 by JanL (Talk | contribs) (Evidence for presence of protein fusion)

Plac+fused PDC-ADH

lac promoter plus PDC-ADH fusion protein for ethanol production


Generation of protein fusion

Bioethanol1.jpg

Bioethanol2.jpg

Evidence for presence of protein fusion

DNA level evidence


Bioethanol3.jpg

File:Bioethanol sequencing.jpg


Protein level evidence


Bioethanol4.jpg

Bioethanol5.jpg

Bioethanol6.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1109
    Illegal AgeI site found at 2315
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

No part name specified with partinfo tag.

File:Bioethanol7

Activity of adhB as a fusion member BBa_K1122674 Activity of adhB as a fusion member Not understood

File:Bioethanol8

Microscopy of cells expressing fused protein BBa_K1122674 Microscopy of cells expressing fused protein Not understood

File:Bioethanol9

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