Part:BBa_K1149004

pelB-pueB expression

PUR degradation enzyme from Pseudomonas chlororaphis.

Characterisation

Our PueB construct was tested for growth upon induction by addition of Xylose.

PueB growth assay. E. coli (MG1655) transformed with PueB BBa_K1149004 were grown with either 0% or 2% Xylose to induce PueB expression. There was no difference between no induction and induction as a t-test gave p = 0.6040 > 0.05. Growth was at 37°C in LB with shaking. Error bars are SEM, n=4. Figure made by Imperial College London 2013 iGEM.

Conclusion: There is no growth inhibition caused by induction in PueB.

References:

[http://www.sciencedirect.com/science/article/pii/S0964830501000427 Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis (2001)]

[http://www.sciencedirect.com/science/article/pii/S0964830502000690 secretion and expression in E.coli]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 99
  Illegal BglII site found at 1213
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 407
  Illegal AgeI site found at 752
  Illegal AgeI site found at 1241
  Illegal AgeI site found at 1517
  
• 1000
  COMPATIBLE WITH RFC[1000]