Coding

Part:BBa_K1111013:Experience

Designed by: Sandra Elisabeth Chaudron, Caroline Desmurget and Mareike Apelt   Group: iGEM13_EPF_Lausanne   (2013-09-19)
Revision as of 10:50, 2 October 2013 by Toone (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1111013

As said in the main page, this part is a negative control with a lower Kd compared to BBa_K1111012 and BBa_K1111014.

Sequencing

We sequenced this part once the Gibson assembly made. To do so, we used primers for iGEM sites VF2 and VR, in order to sequence all that was inserted in the backbone pSB1C3. The sequencing results are shown here :

Microscopy

Also, we transformed cells and used them to do microscopy. Two experiments were done, one with a fluorescent antibody against streptavidin and the other one with a fluorescent biotin. We did both in case the antibody would be too big, since biotin is a small molecule best suit to cross glycocalix.


User Reviews

UNIQfa1a6aff89231513-partinfo-00000000-QINU UNIQfa1a6aff89231513-partinfo-00000001-QINU