Regulatory
Part:BBa_K1151002:Design
Designed by: Emanuele Rizzo Group: iGEM13_UniSalento_Lecce (2013-09-21)
Revision as of 18:08, 2 October 2013 by LeleBiotec (Talk | contribs)
Double divergent promoter pnikR-pexbB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The double divergent promoter was inserted in a pGEM-T Easy vector.
Source
This part comes from H. pylori genomic sequence and it was amplified by PCR.
References
1. Delany et al, In vitro analysis of protein-operator interactions of the NikR and Fur Metal-Responsive Regulators of coregulated genes in Helicobacter pylori. J Bacteriol. 2005 Nov;187(22):7703-15.