Coding
Part:BBa_K1111013:Design
Designed by: Sandra Elisabeth Chaudron, Caroline Desmurget and Mareike Apelt Group: iGEM13_EPF_Lausanne (2013-09-19)
Ice Nucleation Protein fused to Streptavidin Dead
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1727
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1727
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1727
Illegal NgoMIV site found at 1036
Illegal AgeI site found at 1691
Illegal AgeI site found at 1742 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Gibson Assembly
Source
Can be expressed in Escherichia Coli